50X TAE Buffer - Tris Acetate EDTA Buffer, 500 mL
50X TAE Buffer (Tris-Acetate-EDTA) is a concentrated stock solution widely used for DNA and RNA electrophoresis. It can be easily diluted to 1X or 0.5X working strength depending on the needs of the experiment.
Tris helps maintain a stable pH during electrophoresis, acetate provides the counter ion for current flow, and EDTA binds divalent cations like MgĀ²āŗ, protecting nucleic acids from nuclease activity.
Formulation (1X working solution): 40 mM Tris, 20 mM Acetic acid, 1 mM EDTA, prepared with ultrapure 18.2 MĪ© water.
Safety Data Sheet (SDS) and Certificate of Analysis (COA) - available
- Expiration date: 2 years from manufacturing date
100% Completely Sterile:
Commonly used Applications:
- Supports separation and visualization of nucleic acids with consistent migration patterns
- Provides EDTA-mediated protection of DNA and RNA from nuclease degradation
- Ensures reliable electrophoresis performance across extended run times
CAUTION: Research use only.
Product Properties
| Property | Value |
|---|---|
| Concentration | 50X |
| pH (1X solution) | ~8.3 at 25 Ā°C |
| Filtration | Sterile-filtered, 0.22 Āµm |
| Form | Clear, colorless liquid |
| Storage Temperature | Ambient |
Original: $40.00
-70%$40.00
$12.00Product Information
Product Information
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Description
50X TAE Buffer (Tris-Acetate-EDTA) is a concentrated stock solution widely used for DNA and RNA electrophoresis. It can be easily diluted to 1X or 0.5X working strength depending on the needs of the experiment.
Tris helps maintain a stable pH during electrophoresis, acetate provides the counter ion for current flow, and EDTA binds divalent cations like MgĀ²āŗ, protecting nucleic acids from nuclease activity.
Formulation (1X working solution): 40 mM Tris, 20 mM Acetic acid, 1 mM EDTA, prepared with ultrapure 18.2 MĪ© water.
Safety Data Sheet (SDS) and Certificate of Analysis (COA) - available
- Expiration date: 2 years from manufacturing date
100% Completely Sterile:
Commonly used Applications:
- Supports separation and visualization of nucleic acids with consistent migration patterns
- Provides EDTA-mediated protection of DNA and RNA from nuclease degradation
- Ensures reliable electrophoresis performance across extended run times
CAUTION: Research use only.
Product Properties
| Property | Value |
|---|---|
| Concentration | 50X |
| pH (1X solution) | ~8.3 at 25 Ā°C |
| Filtration | Sterile-filtered, 0.22 Āµm |
| Form | Clear, colorless liquid |
| Storage Temperature | Ambient |
